Laboratory DNA/RNA Extraction System Technology

Laftech Bio-Cabinets supply quality laboratory equipment and products throughout Australia. Euro Clone’s Duplica Prep Automated DNA/RNA Extraction System, Blood DNA, Viral RNA/DNA and Body Fluid Kits complement our range of contamination control, fume extraction, forensic and other laboratory equipment and consumables.

The Duplica Prep is an automated nucleic acids purification system that allows to obtain DNA and RNA from different specimens types. Duplica Prep. is a IVD CE “walk away” equipment that provides pure nucleic acids with a high yield of recovery suitable for diagnostic and research purposes. Duplica Prep. platform avoids cross contamination problems, by the use of disposable tips, which prevent the contact with biological samples.

Duplica Prep Key Features

• Bench top instrument with small footprint
• Sample volume up to 600 μl
• 1-12 samples per run
• Duplica Prep  Kits and protocols for a wide range of sample materials
• Standard plastic disposables, quantitative yield, highest purity
• Integrated buffer dispensing
• Revolutionary resuspension technology
• Barcode reading available
• Processing time > 30 min
• Weight 45 kg
• Dimensions 620x520x550 mm (LxWxH)
• Power Supply 110-230

Duplica Prep Overview

Principle of functioning

The principle of functioning of the Duplica Prep platform is based on the nucleic acids separation through the use of magnetic beads. The system has been developed during several years through the collaboration with clinical diagnostic laboratories.

The platform operates in association with the panel of reagents Duplica Extraction Kit, this synergy allows to obtain, with good yield, nucleic acids (DNA and RNA) suitable for molecular biology applications. The platform includes a barcode reader useful for a better samples traceability.

The processing time is variable between 40 - 75 minutes depending on the extraction matrices. The number of samples processed varies from 1 to 12 without reagents wasting.

The magnetic separation is made through the use of 12 metal rods that are magnetized by an external magnet. The magnetization is required to capture the beads and to transfer them into the subsequent solutions. During the steps in which the rods are not magnetized, the rods play an efficient role of beads resuspension into the extraction solution (washing and elution solutions). The resuspension is made by the rods rotation at high speed. This fast and efficient phase, plays a major role in obtaining good yield and quality of nucleic acids (DNA and RNA) (see picture 1).

It’s finally relevant to underline that, the use of Duplica Prep’s technology allows to avoid liquid transfer, since the system captures and moves the beads from one buffer to another. This feature, together with the use of disposable tips, allows to virtually prevent any contamination problem.

Example of magnetization procedure and metal rods rotation.

The Duplica Prep extraction platform consists on the components listed and summarised below.

Duplica Prep extraction platform, internal graphic diagram.

A - Magnet and engine for magnetization and rotation of the metal rods.
B - Buffers dispensing unit (8 different buffers).
C - 96 Deep Well Plate and Tip and Tube Rack positioning truck for placement of disposable tips.
D - Control unit.
E – 12 magnetisable metal rods.
F - Automatic system for tips elimination.
G - Optical barcode reader (supplied together with the equipment).

Technical Features for the Duplica Prep Automated DNA/RNA Extraction System:

Principle: extraction by magnetic beads
Platform type: walk away
Processing time: 40 - 75 min (depending on the samples).
Samples number: 1 - 12 (with no reagents waste)
Sample volume: 100 - 600 μL
Weight: 45 Kg (100 lbs)
Dimensions W x D x H: 620 x 520 x 550 mm
Power supply: 110 - 230 VAC / 50-60 Hz
Power Consumption: 2 kVA
Fuse: 10 A (inert)
Air Pressure: no

Emissions
Noise: <80 dB
Gas, waste, vapors: no

Environmental Conditions of Use
Environmental temperature: 15 - 35 ° C
Relative Humidity: 15 - 75% (no condensation)

Compliance:
Duplica Πrep extraction platform follows the directive 98/79/EC on medical devices - in vitro diagnostic.

Installation space required

Duplica Prep extraction platform is a bench equipment with small dimensions and doesn’t need any special installation requirements.

Below the service areas required:
• 10 cm rear, to allow optimal ventilation.
• 25 cm to the platform right, necessary for Duplica Extraction Kit positioning.

Generic Features for the Duplica Prep Automated DNA/RNA Extraction System:

No Cross Contamination
Duplica Prep extraction platform presents no cross contamination problems, because the equipment components are never in contact with the biological samples. The magnetic rods are protected through the use of a single-use special tips, patented for this platform.

Walk Away System
Each extraction set up requires an hands-on part, concerning the positioning of the 96 Deep Well Plate (supplied together with the extraction reagents) and Tip and Tube Rack for tips loading, on the internal truck. After the extraction start, the operator intervention is no required until the end of the extraction procedure.

Lack of Inhibition Problems
The reagents associate to the Platform include 4 different washing buffers. Several washing steps are performed in order to guarantee the elimination of inhibition problems.

Extraction Control
During the extraction set up, the system allows the inclusion of an extraction control, if required by the operator.

Duplica Formats Available

EKI001 Duplica Prep
Intended Use: Automated DNA/RNA extraction system for biological fluids.

EKI002250 Duplica Blood DNA Kit (180 Extractions)
Intended Use: For isolation of human DNA from human whole blood samples suitable for subsequent in vitro diagnostic purposes.
Material Supplied:
Magnetic Beads: 30 ml
Lysis Buffer (blood): 120 ml
Lysis Buffer (plasma): -
Binding Buffer (blood): 240 ml
Binding Buffer (plasma): -
Wash Buffer 1: 200 ml
Wash Buffer 2: 200 ml
Wash Buffer 3: 200 ml
Wash Buffer 4: 200 ml
Elution Buffer: 80 ml
Protease: 2 ml
Poly(A) RNA: -
Poly(A) RNA Buffer: -
Disposable Tips: 180 pcs
2 mL Deep Well Plates: 15 pcs
0.75 mL Reaction Tubes: 360 pcs
0.75 mL Caps: 180 pcs


EKI003200 Duplica Viral RNA/DNA Kit (180 Extractions)
Intended Use: For isolation of bacterial and viral nucleic acids from plasma and serum, urine, liquor and also for different kind of swabs and feces suspensions suitable for subsequent in vitro diagnostic purposes.
Material Supplied:
Magnetic Beads: 30 ml
Lysis Buffer (blood): -
Lysis Buffer (plasma): 45 ml
Binding Buffer (blood): -
Binding Buffer (plasma): 130 ml
Wash Buffer 1: 110 ml
Wash Buffer 2: 110 ml
Wash Buffer 3: -
Wash Buffer 4: -
Elution Buffer: 30 ml
Protease: 2 ml
Poly(A) RNA: 2 x 350 μg
Poly(A) RNA Buffer: 2 x 440 μL
Disposable Tips: 180 pcs
2 mL Deep Well Plates: 15 pcs
0.75 mL Reaction Tubes: 360 pcs
0.75 mL Caps: 180 pcs


EKI004200 Duplica NA Body Fluid Kit (180 Extractions)
Intended Use: For isolation of human DNA from human whole blood samples or bacterial and viral nucleic acids from different kinds of body fluids such as: whole blood, plasma, serum, urine, liquor and also for different kind of swabs and feces suspensions suitable for subsequent in vitro diagnostic purposes.
Material Supplied:
Magnetic Beads: 30 ml
Lysis Buffer (blood): 95 ml
Lysis Buffer (plasma): 2 x 48 ml
Binding Buffer (blood): 210 ml
Binding Buffer (plasma): 210 ml
Wash Buffer 1: 210 ml
Wash Buffer 2: 110 ml
Wash Buffer 3: 110 ml
Wash Buffer 4: 110 ml
Elution Buffer: 50 ml
Protease: 2 ml
Poly(A) RNA: 2 x 350 μg
Poly(A) RNA Buffer: 2 x 440 μL
Disposable Tips: 180 pcs
2 mL Deep Well Plates: 15 pcs
0.75 mL Reaction Tubes: 360 pcs
0.75 mL Caps: 180 pcs

Duplica Applications

Duplica Prep, when used in combination with its dedicated extraction kits, is suitable for the isolation of nucleic acids from whole blood, EDTA - plasma, serum, swabs of different origins (vaginal, urethral, rectal, et al), feces, urine, enrichment culture, transport medium, liquor, amniotic fluid, BAL and sputum.

EuroClone Laboratory Equipment.

Laftech Bio-Cabinets are the exclusive distributor of EuroClone Duplica Prep in Australia. We are justifiably proud of our record for professional service and encourage you to consider our company the next time you're looking to buy or upgrade lab equipment. Our associated company Laftechnologies is a NATA accredited laboratory providing decontamination, testing, certification and back-up servicing for food technology and other related laboratory equipment.

For more information about specifications and related products > Download 692kb < the Bio-Cabinets Duplica Prep Automated DNA/RNA Extraction System EuroClone data sheet.

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Laftech Bio-Cabinets - Australian Laboratory supplier of Automated DNA/RNA extraction systems, Blood DNA, Viral RNA/DNA and Body Fluid Kits.